peration method
1. Collect blood at 0.5-1ml, 1000 rpm and centrifuge for 3 minutes to separate the serum. It can also be left overnight at 4 ° C to allow the serum to precipitate naturally.
2. Take out the test card (if refrigerated, you need to return to room temperature and then open the package). After unpacking, place it on the table, and slowly and accurately add 2 drops of serum to the sample well with a dropper.
3, 10-15 minutes to judge the results, the results after 15 minutes are invalid.
Result judgment
1. Positive: In the observation hole, purple lines appear in both the test line area (T) and the control line area (C). The higher the titer of Toxoplasma gondii antibodies, the darker the detection line (T).
2. Weak positive: In the observation hole, the magenta line appears in the test line area (T) and control line area (C), but the color in the test line area is very light.
3. Negative: In the observation hole, only one purple line appears in the control line area (C).
4. Fault: In the observation hole, the magenta line does not appear in the control line area (C) or the detection line area (T); Or only the magenta line appears in the detection line area (T).
Precautions
1. Products with damaged aluminum foil bags cannot be used. The test card must be used within 1 hour after removal.
2. If the collected serum sample cannot be detected immediately, it should be stored at 4 ℃ for short-term storage (48 hours), and -20 ℃ for long-term storage.
3. When the sample is added to the “sample well”, foaming should be avoided. The amount of sample should be controlled at about 100ul (about two drops of sample). Excessive sample addition does not increase detection sensitivity.
4. The level of antibody is only related to the depth of the detection line (T line), and has no correlation with the depth of the quality control line (C line). As long as the quality control line is clearly visible, the experiment is valid.
5. When the content of test substance is very high, after the test line appears, the red color will gradually fade and even disappear. At this time, it is recommended to dilute the sample with saline several times before testing, and the red line can be stabilized.
6. Used test cards should be properly disposed of in accordance with local pollutant disposal regulations.
7. Products that have expired cannot be used.
8. This product is a disposable product, please do not use it again.
